Genome-wide identification and transcriptional characterization of DNA methyltransferases conferring temperature-sensitive male sterility in wheat

Abstract Background DNA methyltransferase (DMT) genes contribute to plant stress responses and development by de novo establishment subsequent maintenance of methylation during replication. The photoperiod and/or temperature-sensitive genic male sterile (P/TGMS) lines play an important role in hybrid seed production wheat. However, only a few studies have reported on the effect DMT sterility Although been investigated some species, identification analysis wheat ( Triticum aestivum L.) based genome-wide levels not reported. Results In this study, detailed overview phylogeny 52 (TaDMT) was presented. Homoeolog retention for TaDMT significantly above average rate whole-wheat genes, indicating functional importance many homoeologs. We found that strikingly high number resulted mainly from significant expansion TaDRM subfamily. Intriguingly, all 5 paralogs belonged DRM subfamily, we speculated tandem duplications might crucial subfamily expansion. Through transcriptional TGMS line BS366 its hybrids with other six fertile under conditions, concluded TaCMT-D2 , TaMET1-B1 TaDRM-U6 be involved BS366. Furthermore, correlation showed negatively regulate expression TaRAFTIN1A gene pollen development, so regarding epigenetic regulatory mechanism underlying via interaction between . Conclusions Our findings presented phylogenic could provide novel insights into effects